The non-specific lipid transfer protein from hazelnut, Cor a 8, a relevant food allergen
نویسندگان
چکیده
Methods nCor a 8 was purified from raw hazelnuts by precipitation and chromatographical steps. Subsequently, the protein was identified by N-terminal sequencing and mass spectrometry. Stability of the protein was investigated by simulated gastric and duodenal digestion assays. Hazelnut lipids were extracted employing the hexane method. Finally, IgE binding activity of native and reduced/alkylated nCor a 8 was tested in ELISA using sera from 6 hazelnut allergic patients.
منابع مشابه
Structural and Functional Characterization of the Hazelnut Allergen Cor a 8
Nonspecific lipid transfer proteins (nsLTPs) are basic proteins, stabilized by four disulfide bonds, and are expressed throughout the plant kingdom. These proteins are also known as important allergens in fruits and tree nuts. In this study, the nsLTP from hazelnuts, Cor a 8, was purified and its crystal structure determined. The protein is stable at low pH and refolds after thermal denaturatio...
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